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Figure 3


Fig. 3. Marker analysis of the cleavage regions positive for UBPY, AMSH and ubiquitylated proteins. (A-A'') HeLa cells were transfected with HA-Ub, and triple-stained with anti-HA antibody (A), anti-Aurora B antibody (A', red) and TO-PRO-3 (A', blue) at stage 2 of cytokinesis. (B-B'') HeLa cells at stage 2 of cytokinesis were triple-stained with FK2 (B), anti-MKLP1 antibody (B', red) and TO-PRO-3 (B', blue). (C-C'') HeLa cells at stage 4 of cytokinesis were triple-stained with anti-UBPY antibody (C), anti-Aurora B antibody (C', red) and TO-PRO-3 (C', blue). (D-D'') HeLa cells at stage 2 of cytokinesis were triple-stained with anti-AMSH antibody (D), anti-MKLP1 antibody (D', red) and TO-PRO-3 (D', blue). (E-E'') HeLa cells at stage 4 of cytokinesis were double-stained with anti-Hrs (E) and anti-tubulin (E') antibodies. Cytoplasmic punctate staining in green indicates endosomes. A''-E'' are merged images. Insets show high-magnification images of the central spindle/midbody regions. Scale bars: 10 µm.





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