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Fig. 2. Centrosome and/or spindle-pole localization of P-Tyr421-cortactin, P-Tyr466-cortactin and P-Tyr482-cortactin in mitotic 3T3-L1 cells. (A) Double-immunofluorescence staining of cortactin using ${alpha}$ -tubulin or actin antibodies. (B) Double-immunofluorescence staining using anti-P-Tyr421-cortactin (PY421C), anti-P-Tyr466-cortactin (PY466C) or anti-P-Tyr483-cortactin (PY482C) antibodies and anti- ${gamma}$ -tubulin antibody ( ${gamma}$ -Tu). (C) Double-immunofluorescence staining using anti-P-Tyr421-cortactin (PY421C) or anti-P-Tyr466-cortactin (PY466C) antibodies and anti- ${alpha}$ -tubulin ( ${alpha}$ -Tu) antibody (D) Phosphotyrosine proteins and spindle poles. Metaphase 3T3-L1 cell was stained with anti-phosphotyrosine antibody (PY), anti- ${gamma}$ -tubulin antibody ( ${gamma}$ -Tu) and DAPI. D, DAPI; C, anti-cortactin antibody; A, anti-actin antibody; ${alpha}$ -Tu, anti- ${alpha}$ -tubulin antibody; M, the merged picture of panels D, C and A (or ${alpha}$ -Tu). Scale bars: 10 µm.

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