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Fig. 3. The dynamic association of P-Tyr-cortactin with centrosome or spindle poles during mitosis in 3T3-L1 cells. (A) Association of P-Tyr421-cortactin with spindle poles in mitotic phases. Nuclei were stained with DAPI (blue), anti-P-Tyr421-cortactin (red) and anti- ${alpha}$ -tubulin (green) antibodies. The insertion in the G2-M panel is an enlarged spindle pole or centrosome. (B) Dynamic association of P-cortactin with centrosomes or spindle poles. Arrowheads indicate the staining of centrosomes by using anti- ${gamma}$ -tubulin antibody ( ${gamma}$ -Tu) and P-Tyr421-cortactin (PY421C). Insertions are enlarged views of the centrosomes. (C) Spindle poles. Staining was for P-Tyr421-cortactin (PY421C) and anti- ${alpha}$ -tubulin ( ${alpha}$ -Tu). (D) Localization of P-Tyr421-cortactin, P-Tyr466-cortactin and P-Tyr482-cortactin in pericentriolar material. Cells expressing transfected GFP tagged centrin 2 (GFP-Cetn2) were stained with anti-P-Tyr421-cortactin, P-Tyr466-cortactin or P-Tyr482-cortactin antibodies. GFP-Centrin 2 was visualized by eGFP fluorescence. Scale bars: 10 µm.

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