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First published online April 3, 2008

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Making and breaking cell-cell contacts

The transmembrane proteins CD47 and SHPS-1 can interact specifically via their respective extracellular domains, leading to the direct association of CD47- and SHPS-1-expressing cells. Although cell-to-cell communication via CD47 and SHPS-1 has a role in diverse processes, including the formation of neurites on neurons, how the CD47–SHPS-1 interaction is regulated is unknown. On page 1213, Takashi Matozaki and colleagues use two populations of CHO cells, which express either CD47 or SHPS-1, to investigate the regulation of the CD47–SHPS-1 system. The authors show that the association of the two proteins, and hence the two cell types, causes the trans-endocytic uptake of CD47 into SHPS-1-expressing cells (SHPS-1 is also trans-endocytosed by CD47-expressing cells, but to a lesser extent). The authors show that the trans-endocytosis of CD47 is mediated by clathrin and dynamin, and requires the juxtamembrane region of SHPS-1. After trans-endocytosis, CD47 colocalises with SHPS-1 in multivesicular bodies, implying that the two proteins are trans-endocytosed together and are subsequently degraded within lysosomes. Importantly, inhibiting the trans-endocytosis of CD47 stimulates the aggregation of CD47- and SHPS-1-expressing cells. The authors conclude that the trans-endocytosis of CD47 is a key regulator of the CD47–SHPS-1 system.

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Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47–SHPS-1 system

Shinya Kusakari, Hiroshi Ohnishi, Feng-Jie Jin, Yuka Kaneko, Takaaki Murata, Yoji Murata, Hideki Okazawa, and Takashi Matozaki
JCS 2008 121: 1213-1223. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content