QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online April 3, 2008

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

Trephin takes up the yolk

During oogenesis in the mosquito, the ingestion of blood stimulates vitellogenesis – the uptake and storage of yolk by the oocyte. The yolk proteins vitellogenin and lipophorin are rapidly endocytosed via specific transmembrane receptors, both of which are members of the LDL-receptor superfamily. In mammals, such receptors are internalised by a clathrin-mediated route in which phosphotyrosine-binding (PTB) domain proteins such as ARH dock at the receptor's cytosolic FxNPxY motif, but it is not known whether a similar mechanism exists in the mosquito. On page 1264, Linton Traub and colleagues identify a mosquito protein, which they term trephin, that is orthologous to ARH. Similar to ARH, trephin binds to FxNPxY motifs, and also to clathrin and PtdIns[4,5]P₂. Trephin also binds to the clathrin adaptor AP-2; however, unlike ARH and other mammalian PTB proteins (which bind to the -platform subdomain), trephin binds to the β-platform subdomain of AP-2, indicating that a switch in domain specificity has occurred during evolution. Importantly, the expression of trephin is strongly upregulated in the oocyte following a blood meal, which is consistent with a role in the endocytosis of yolk proteins. These results elucidate a novel mechanism of clathrin-mediated endocytosis in the mosquito.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Internalization of LDL-receptor superfamily yolk-protein receptors during mosquito oogenesis involves transcriptional regulation of PTB-domain adaptors

Sanjay K. Mishra, Anupma Jha, Amie L. Steinhauser, Vladimir A. Kokoza, Charles H. Washabaugh, Alexander S. Raikhel, Woodbridge A. Foster, and Linton M. Traub
JCS 2008 121: 1264-1274. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?