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Fig. 1. Depletion of 
E-catenin has no major effect on interaction between β-catenin, N-cadherin and other β-catenin-binding proteins. (A,B) Total protein lysates from E14.5 wild-type (WT) and E-catenin–/– (knockout, KO) mouse brains were immunoprecipitated with control (IgG) or anti-β-catenin (β-cat) antibodies and the resulting protein complexes were separated by SDS-PAGE and stained with Colloidal Blue and Silver stain (A) or analyzed by western blot with anti-E-catenin, N-cadherin or β-catenin antibodies (B). Note that although E-catenin becomes depleted from β-catenin protein complexes, composition or relative abundance of other proteins does not change. Western blotting reveals no significant changes in the association between β-catenin and N-cadherin. (C-D") Despite disruption of apical junctional complexes and loss of cell polarity, β-catenin continues to colocalize with N-cadherin at the periphery of E-catenin–/– neural progenitor cells. Cortical sections from E13.5 wild-type (WT) and E-catenin–/– (KO) embryos were stained with anti-N-cadherin (N-cad) and anti-β-catenin (β-cat) antibodies. Scale bar: 15.9 µm.
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