spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Gβ{gamma} and G{alpha}q subunits dissociate upon carbachol stimulation in the presence of Cav1. (A) Pseudo-colored FRET images illustrating the time-dependence of energy transfer from eCFP-Gβ{gamma} to G{alpha}q-eYFP in acetylcholine (5 µM)-stimulated FRTcav+ (upper panel) and FRTcav– (lower panel) cells. The FRET color-scale is shown for comparison (red, 100%; black, 0%). For both series, the first panel was recorded before stimulation and the elapsed time between each image is 20 seconds. (B) Compiled results of the changes in NFRET from eCFP-Gβ{gamma} to G{alpha}q-eYFP in unstimulated and acetylcholine (5 µM)-stimulated FRTcav+ and FRTcav– cells with time. Studies were done using nine (FRTcav–) and four (FRTcav+) independent sets of samples (each measured in triplicate and averaged). (C) Pseudo-colored NFRET image illustrating energy transfer between free eCFP and eYFP.





Right arrow Return to article