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Figure 4


Fig. 4. The presence of Cav1 causes a sustained Ca2+ response. Change in intracellular Ca2+ in 1-ml suspensions of 106 FRTcav+ or FRTcav– cells transfected with either empty vector, PLCβ1 or PLCβ2, and loaded with the Ca2+-sensitive dye FURA-2AM upon addition of 1 µM acetylcholine (see Materials and Methods). n=6 of triplicate sets of samples. Black arrows indicate time of stimulus addition.





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