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Figure 5


Fig. 5. Immunofluorescence confocal images showing the colocalization of Cav1 and G{alpha}q. Confocal images of fixed FRT cells expressing transiently transfected Cav1-eGFP (green) are immunostained for endogenous G{alpha}q (red). Yellow pixels in the merged images indicate colocalization. Cells were either fixed without stimulation (top) or at 4 minutes after stimulation with 5 µm carbachol (bottom). Images were recorded with emission filter sets BP505-530 (green) and LP650 (red) using two separate lasers for sequential excitation (488 nm for green and 633 nm for red).





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