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Fig. 6. FRET illustrates that activated G ${alpha}$ _q has a stronger affinity for Cav1-eGFP than do Gβ ${gamma}$ subunits. (A) The titration curve of XRITC-Gβ ${gamma}$ and XRITC-G ${alpha}$ _q(GTP ${gamma}$ S), showing the normalized decrease in Cav1-eGFP intensity as the G proteins are added, caused by energy transfer (FRET). (B) Bar graph showing the values of the normalized donor intensities for free acceptor XRITC at 60 nM (n=2), XRITC-Gβ ${gamma}$ at 70 nM (n=3), XRITC-G ${alpha}$ _q(GDP) (n=3), XRITC-G ${alpha}$ _q(GTP ${gamma}$ S) (n=5), XRITC-G ${alpha}$ _q(GTP ${gamma}$ S) + 80 nM PLCβ1 (n=2) and XRITC-G ${alpha}$ _q (GTP ${gamma}$ S) starting with threefold the initial amount of Cav1-eGFP-containing FRTcav^– membranes (n=3). Asterisks indicate significant difference, P<0.001, by one-way variance from control.

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