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Figure 1


Fig. 1. Satellite cells express β-catenin. (a-f) Immunostaining of EDL myofibres immediately after isolation (a) showed that some Pax7+ satellite cells (green) expressed nuclear β-catenin (red). Similarly, once activated (T24), β-catenin could be found in the nucleus of some satellite cells (b). After the first division (T48) β-catenin was localised in the nucleus (c) and/or at the cell surface (d). When satellite cells began to differentiate or self-renew (T72), fewer were found with nuclear localised β-catenin (e,f). (g-i) To test the retroviral vectors, satellite cells were infected with either (g) pMSCV–β-catenin–IRES–eGFP or (h) pMSCV–ST-β-catenin–IRES–eGFP. Immunostaining for eGFP (green) and β-catenin (red) revealed that infected satellite cells robustly co-expressed these proteins, compared to control (pMSCV-IRES-eGFP) (i). Nuclei were counterstained with DAPI.





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