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Fig. 3. Constitutively expressed β-catenin inhibits myogenic differentiation and enhances self-renewal of satellite cells. (a-l) Plated satellite-cell-derived myoblasts were infected, switched to low-mitogen medium 24 hours later, cultured for at least another 4 days, fixed and immunostained. Only few satellite cells with retrovirally encoded wild-type or stabilised β-catenin (eGFP+, green) contained MyoD (a,b; red), myogenin (d,e; red) or MyHC protein (g,h; red), in contrast to control-infected cultures, which contained many multinucleated myotubes immunostaining for (c) MyoD, (f) myogenin and (i) MyHC. By contrast, Pax7 protein (red) was present in many satellite cells infected with (j) pMSCV–β-catenin–IRES–eGFP (catenin-RV) or (k) pMSCV–ST-β-catenin–IRES–eGFP (ST-β-catenin-RV), whereas (l) control-infected (pMSCV-IRES-eGFP) satellite cells only had the occasional unfused cell staining for Pax7. Nuclei were counterstained with DAPI (blue). (m) Quantification of experiments shown in a-l. Values are population mean ± s.e.m. from at least ten random fields. *P<0.05, significantly different from control cultures.
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