|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 4. β-catenin increases the proportion of C2 reserve cells. When C2 cells are induced to differentiate by culture in low-mitogen medium, most respond by differentiating, but some down-regulate MyoD, express Pax7 and exit the cell cycle, entering a quiescent-like state to become reserve cells (Yoshida et al., 1998; Olguin and Olwin, 2004). (a-l) Infection of proliferating C2 cells with retrovirus encoding (a) wild-type (β-catenin-RV) or (b) stabilised β-catenin (ST-β-catenin-RV) and subsequent culture in low-mitogen medium resulted in many infected eGFP+ (green) cells with Pax7 (red). Cells infected with (c) control pMSCV-IRES-eGFP, however, mostly responded by differentiating and fusing into large multi-nucleated myotubes; and Pax7 was restricted to the occasional reserve cell. As with primary satellite cells, most C2 cells that constitutively expressed β-catenin (eGFP, green) did not contain MyoD (d,e; red), myogenin (g,h; red) or MyHC protein (j,k; red). Control-infected cells formed myotubes that showed robust (f) MyoD, (i) myogenin and (l) MyHC expression. Nuclei were counterstained with DAPI (blue). (m) Quantification of experiments shown in a-l. Values are mean ± s.e.m. from at least ten random fields. *P<0.05, significantly different from control cultures.
|
