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Figure 8


Fig. 8. Repressing β-catenin transcriptional activity enhances myogenic differentiation. (a,b) Myofibre-associated satellite cells infected with pMSCV–β-catenin–ERD–IRES–eGFP (β-catenin–ERD–RV) had significantly more eGFP+ (green) cells expressing myogenin (red) compared with (b) pMSCV-IRES-eGFP-infected (control-RV). Co-immunostaining for eGFP and Pax7, or eGFP and MyoD proteins revealed that fewer satellite-cell progeny that expressed β-catenin–ERD contained either Pax7 or MyoD (quantified in c). (d,e) Transcriptional repression of β-catenin target genes by β-catenin–ERD enhanced myogenic differentiation, with myotubes with a higher fusion index than control-infected myotubes (quantified in f). Nuclei were counterstained with DAPI (blue). (c,f) Quantification of experiments shown in a,b (c), and in d,e (f). Values are population mean ± s.e.m. *P<0.05, significantly different from control cultures.





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