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Figure 6


Fig. 6. Genetic interaction between slk1 and spo3 alleles. (A) spo3-S3 and spo3-GFP alleles enhance the sporulation defect caused by the deletion of slk1. Homothallic wild-type (S1478), slk1{Delta} (S1883), spo3-S3 (S1734), slk1{Delta} spo3-S3 (S1889), spo3-GFP (S1458) and slk1{Delta} spo3-GFP (S1888) strains were sporulated on MEA plates, and DIC images were taken after 2 days of incubation at 25°C. (B) MEA plates from the experiment shown in A were stained with iodine vapour after incubation at 25°C for 3 days. Staining is slightly reduced in the slk1{Delta} single mutant, but it is completely abolished in the double mutants. (C) Psy1-GFP fails to encapsulate the nuclei in slk1{Delta} spo3-S3 cells. Mutant cells carrying the plasmid pREP81-Psy1-GFP were sporulated on MEA plates at 25°C, stained with Hoechst and photographed. Merged images are shown in the right column. (D) Forespore membranes are unable to engulf the nuclei in slk1{Delta} spo3-GFP cells. Forespore membranes were visualized by Spo3-GFP fluorescence and DNA was stained with Hoechst. Merged images are shown in the right column. Scale bars: 10 µm.





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