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Figure 7


Fig. 7. Slk1-GFP localizes to the spindle pole body and the forespore membrane during meiosis. (A) A homothallic strain harbouring the slk1-GFP allele under its own promoter (S1717) was incubated on MEA for 24 hours at 25°C. Meiotic cells were observed under the fluorescence microscope and photographed. Stacks of four images were taken every 2.5 minutes. Deconvolution of images and maximal projections were obtained using Deltavision software. Notice that Slk1 (arrow) moves from the spindle pole body to the forespore membrane (t=0-10 minutes). After 12.5 minutes, Slk1-GFP localizes to the forespore membrane. (B) A homothallic strain expressing Slk1-GFP under the nmt1(41) promoter (S1931) was incubated on MEA for 24 hours at 25°C and photographed. (Ba) Slk1 (arrow) localizes to the spindle pole body in metaphase II cells. (Bb, Bc) At anaphase II, Slk1 extends from the spindle pole body to the forespore membrane. (Bd) After anaphase II, Slk1 localizes to the forespore membrane. Note that, in the mitotic cells shown in the fields photographed, Slk1 localizes to the nucleolus when overexpressed. Scale bars: 4 µm.





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