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Figure 2


Fig. 2. CD44-deficient fibroblasts exhibit less directional migration despite an increased migrational velocity. Cells were grown in 10% FBS and wounded. (A) Efficiency of wound closure in CD44WT (left top) vs CD44KO (right top). Cells (10 CD44KO and 10 CD44WT) were tracked using ImagePro 5.0 software (lower images). Individual tracks represent direction of cell migration as determined by live microscopy. Arrows represent position of cells at zero timepoint. Scale bar: 500 µm. (B) Analysis of 10 representative CD44KO and CD44WT cells and their positional y-coordinates. Convergence of the tracks towards the same y-coordinate indicates that cells have closed the gap. Similarly, as a cell exhibits directional migration, the y-coordinate track is linear, whereas a directionless path reveals a `zig-zag' pattern. (C) Average cell velocity calculated over the course of wound closure. Error bars represent s.e.m. (*P<0.0001). These experiments were repeated three times for each group with similar results.





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