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Figure 3


Fig. 3. Reconstitution of CD44KO cells with CD44 rescues the migrational phenotype. CD44KO fibroblasts were transfected with recombinant CD44 (pRC/pCMV {Delta}1230) using nucleofection. (A) Following reconstitution, cells were stained with anti-CD44 (mAb) (left panel), and phalloidin (middle panel). Merge of CD44 and phalloidin stains (right panel). Arrow indicates CD44KO fibroblast and asterisk a reconstituted (CD44KO.CD44) fibroblast. Scale bar: 50 µm. (B) Reconstitution with CD44 (CD44KO.CD44) decreases the mean velocity. Error bars represent s.e.m. (P<0.002). (C) Measure of instantaneous velocity in CD44WT, CD44KO and reconstituted cells (CD44KO.CD44). (D) Measure of the confinement ratio (distance between start and end point divided by total length of track) in CD44WT, CD44KO and reconstituted (CD44KO.CD44) cells. A ratio of 1.0 indicates that the cell is moving in a straight line. Data in B, C and D are representative of one experiment performed three times with similar results.





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