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Figure 6


Fig. 6. Active TGFβ partially rescues the phenotype of CD44KO fibroblasts. (A) CD44KO fibroblasts were grown on coverslips and treated with either latent or active TGFβ1 (0.02 nM) for 24 hours, then stained for F-actin and compared with untreated CD44KO and WT fibroblasts. Scale bar: 40 µm. (B) Quantification of fluorescent intensity across the lines shown in the corresponding panels in A (above), to indicate stress fiber density, using Image Pro software. Asterisks demarcate cells being quantified in A and their corresponding line graphs in B. (C) Instantaneous velocity of CD44WT, CD44KO and CD44KO cells treated with active TGFβ. (D) Measure of the confinement ratio (distance between start and end point divided by total length of track) in CD44WT, CD44KO and CD44KO cells treated with active TGFβ. A ratio of 1.0 indicates that the cell is moving in a straight line. Data in A, C and D are representative of one experiment performed three times with similar results.





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