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Fig. 7. Anti-TGFβ antibody reduces and alters stress fiber architecture in CD44WT fibroblasts. (A) CD44WT and CD44KO fibroblasts were grown on coverslips and treated with anti-TGFβ antibody (5 µg/ml) for 24 hours, then stained for F-actin and compared with untreated CD44WT and CD44KO fibroblasts. Scale bar: 20 µm. (B) Quantification of fluorescent intensity across the lines shown in the corresponding panels in A (above), to indicate stress fiber density, using Image Pro software. In the CD44KO and CD44KO + anti-TGFβ panels, two different cells are being quantified in each. Asterisks and triangular shapes demarcate cells being quantified in A and their corresponding line graphs in B. Data are representative of one experiment performed three times with similar results.
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