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Fig. 6. A model for Miranda localization by Myosin II and Myosin VI. (A) Late interphase. We propose the model that Myosin II forms an inactive crescent during late interphase (individual green ovals) because aPKC is absent and cannot phosphorylate and inactivate Lgl (not shown). Myosin II binds to Miranda (red) in an apical crescent. PON is still cytoplasmic during interphase (yellow area). (B) Prophase and pro/metaphase. Very early at prophase, aPKC binds to the apical crescent (purple crescent) and activates Myosin II to form microfilaments (connected green ovals) by phosphorylation of Lgl (not shown). Hence, Miranda is excluded from the apical cortex and mobilized to diffuse rapidly throughout the entire cytoplasm filling the nucleus around the time of nuclear envelope breakdown at pro/metaphase (red area). PON is recruited to the cortex (yellow circle) at prophase. (C) Metaphase. Myosin VI (blue) in the basal half of the cell binds to Miranda to either anchor it or to deliver Miranda by short-range transport to a cortical anchor at the basal crescent. PON is `pushed' along the cortex by Myosin II activity to form a basal crescent.
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