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Figure 3


Fig. 3. Organelle association and ultrastructure of the FAG. (A) 3D model of the FAG and the Golgi complex in Vero cells co-stained with TRITC-phalloidin (red) and anti-giantin (green) antibodies. (B) Co-staining with FITC-phalloidin (green) and the mitochondrial marker MitoTracker (red). (C-G) Ultrastructure of the FAG. The FAG is invariably surrounded by mitochondria (C,D), autophagic vacuoles (D), lysosomes (G), the Golgi complex (D,G) and intermediate filaments (arrowheads in G). In some FAGs (E), there are regions containing highly ordered F-actin or actin bundles (F, enlargement of the boxed area shown in E). Autophagic vacuoles (a), lysosome (l), Golgi cisternae (g), peri-Golgi COPI-coated transport carriers (arrows in G) and electron-dense material in the FAG (asterisks in D) are indicated. Scale bars: 10 µm (B), 3 µm (A,C), 300 nm (D,E) and 100 nm (F,G).





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