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Figure 5


Fig. 5. Microtubule involvement in the formation and location of the FAG. (A-C) Vero cells were treated with NZ (30 µM for 6 hours; A, +NZ) or TX (30 µM for 6 hours; A, +TX) alone or subsequently co-incubated with Jpk (50 nM for 6 hours; B,C). (B) In NZ+Jpk-treated cells, no FAG was formed. Instead, numerous F-actin punctae and F-actin amorphous aggregates were dispersed throughout the cytoplasm (also see inset). (C) Similar results were obtained in TX+Jpk-treated cells (also see inset). (D,E) When NZ and TX were washed-out but Jpk remained (D and E, respectively), a FAG was formed. (F) When cells were incubated with Jpk (50 nM for 6 hours) and then with NZ (30 µM for 6 hours), the structural integrity of the FAG (left panel) remained unaltered despite the disruption of microtubules (right panel). (A-F) Cells were stained with anti-β-tubulin antibodies (A and right panel in F) or TRITC-phalloidin (B-E and left panel in F). Scale bars: 10 µm.





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