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Figure 8


Fig. 8. Proteasomes and proteasomal function in the FAG. (A,B) Vero cells containing a FAG (red) co-stained with anti-FK2 antibodies to show (poly)ubiquitylated proteins (A, green) or with anti-C9 antibodies to reveal the presence of proteasomes (B, green), indicating that the FAG contains proteasomes but not polyubiquitylated proteins. (C-F) FAG clearance was delayed in cells treated with lactacystin (Lac; compare E with C and F with D). (G) Proteasome activity measured in clarified lysates from untreated Vero cells (control, c), from cells treated with lactacystin (+Lac, 10 µM), or from cells treated with Jpk (+Jpk, 50 nM) for different times. Results are the mean ± s.d. from three independent experiments. Differences from control significant at P<=0.01 (**) and P<=0.001 (***). Scale bar: 10 µm.





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