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Figure 1


Fig. 1. PN-1 colocalizes with thrombin and controls its activity in vibrissal follicles. (A) X-Gal (blue) staining of cryostat sections from embryonic hair (E18.5) and whisker (E16.5) follicles of PN-1 knockin (KI) mice during early follicle morphogenesis. (B) Immunohistochemistry (brown staining) of follicles during anagen (P23) and catagen (P25) with an antibody against PN-1 (note that black areas are melanin, not staining). (C) X-gal staining of adjacent longitudinal cryostat sections of dissected postnatal follicles from a PN-1 KI mouse at early anagen (P27). (D) Thrombin immunostaining of a longitudinal cryostat section of a full-length early anagen follicle from a wild-type mouse showing the similarity to PN-1 localization (cf. C) in DS and a subset of ORS cells. (E) Comparison of thrombin proteolytic activity with or without addition of recombinant PN-1 (rPN-1; 1 µg/ml) to anagen whisker follicle homogenates from wild-type and PN-1–/– mice. ***Significant difference (P<0.001). (F) Higher magnification of thrombin immunostained follicular bulbs from wild-type and PN-1–/– mice showing thrombin accumulation in the DP in the absence of PN-1. pDP, presumptive dermal papilla; pDS, presumptive dermal sheath; DP, dermal papilla; DS, dermal sheath; ORS, outer root sheath; C, capsula.





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