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Figure 2


Fig. 2. ABBA is enriched in radial glia. (A) Anti-ABBA antibody recognises a single band from NIH3T3 cell lysate corresponding to the GFP-tagged ABBA, and a lower molecular weight protein from mouse whole brain lysate corresponding to endogenous ABBA. Immunohistochemical detection of ABBA protein (red) on sections from E12.5 (B,E) and E14.5 (I,M,Q) mouse embryonic tissue. Double labelling was performed either with RC2 (C,J,R) or Tuj1 (F,N) (green) and colocalisation with ABBA appears yellow in merged images (D,G,K,O,S) and in corresponding higher magnifications (H,L,P,T). At E12.5, the strongest ABBA expression was detected in the hindbrain floorplate where it colocalised with RC2. ABBA was detected in the radial glia fibers throughout the hindbrain region (arrowhead) and was enriched in the marginal zone where the radial glia end feet are located (arrow) (B-D). No colocalisation was observed with Tuj1, which labelled interneuron axons crossing the ABBA-positive spinal cord floorplate (E-H). At E14.5, ABBA colocalises in glial raphe (arrow) with RC2 (I-L) but not with Tuj1 (M-P). ABBA was detected throughout the developing cortex, where it colocalises in radial glia with RC2 (Q-T). cp, cortical plate; fp, floorplate; hb, hindbrain; mz, marginal zone; ra, glial raphe; gf, glial fiber; vz, ventricular zone. Scale bars: 20 µm in B-G, I-K, M-O, Q-S and 10 µm in H,L,P,T.





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