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Figure 1


Fig. 1. X-Gal staining in E14.5 and E16.5 TOP-gal transgenic embryos. (A) X-Gal staining of E14.5 whole embryos shows that cartilage, skull, vertebral column, ribs and long bones are stained positive for X-Gal. (B,C) Chondrocytes in the ribs and forelimb of E14.5 transgenic embryos show strong X-Gal-positive staining. (D-F) In E16.5 embryos, (E) proliferating (red arrowhead) and (F) hypertrophic chondrocytes (black arrow) and osteoblasts (red arrows) in the metaphysis show positive X-Gal staining. The results indicate that β-catenin signaling is active in proliferating and hypertrophic chondrocytes during cartilage development.





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