spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Skeletal growth is reduced in Col2a1-ICAT transgenic mice. (A) Structure of the transgene construct and the priming sites for genotyping the Col2a1-ICAT transgenic mice. (B) PCR results; genotyping of Col2a1-ICAT transgenic mice. Mice 1, 2, 4 and 5 (lanes 1, 2, 4 and 5) are positive for Flag-ICAT transgene expression (P, positive control; N, negative control). (C) Body weight of 2-week-old and 4-week-old transgenic mice and WT littermates. A significant reduction in body weight was observed in Col2a1-ICAT transgenic mice. (D) Radiographic analysis showing the delay in the longitudinal growth of 1-week-old, 2-week-old and 4-week-old Col2a1-ICAT transgenic mice compared with their WT littermates. (E,F) Alizarin Red and Alcian Blue staining showed that skeletal development was relatively normal in 1-day-old new born Col2a1-ICAT transgenic mice. By contrast, a significant reduction in postnatal skeletal growth was observed in 2-week-old Col2a1-ICAT transgenic mice.





Right arrow Return to article