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Figure 1


Fig. 1. LPS inhibits Notch1 transcriptional activity. (A) RAW264.7 cells were transfected for 24 hours with the expression vector for 4xCSL-Luc and Notch1-IC, along with lacZ, and then exposed to the indicated amount of LPS for 24 hours. (B-D) RAW264.7 cells transfected for 24 hours with the indicated combinations of expression vector for 4xCSL-Luc (B), Hes1-Luc (C) or Hes5-Luc (D) and Notch1-IC along with lacZ, and then exposed to 5 µg/ml LPS for 24 hours. The cells were then lysed and assayed for luciferase activity. The activity of the luciferase reporter in each of the samples was then normalized according to the β-galactosidase activity measured in the same sample. (E) The cell lysates were also subjected to immunoblotting analysis with the anti-Hes1 or anti-Hes5 antibody (top panels). THP-1 cells were treated with IL-1 (5 ng/ml) for 16 hours (middle panel). Jurkat T cells were exposed to UV light (60 J/m2) (bottom panel). The cells were then treated with 200 µM SNAP for 8 hours. The cell lysates were subjected to immunoblotting analysis with the anti-Mn-SOD or anti-CD95L antibody. These results represent the means ± average deviation of triplicates from one of three independent experiments.





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