spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. The N-terminus and PDZ domain of DLG-1 are sufficient for membrane association in dlg-1(ok318) and dlg-1(ok318);ajm-1(RNAi) embryos. (A) Diagram displaying DLG-1::GFP deletion constructs and their localization in wild-type and dlg-1(ok318) embryos. (B,F,J) All constructs localized properly along the junctions of epidermal cells in wild-type embryos. (C,G,K) Localization of DLG::GFP deletions in dlg-1(ok318) homozygous mutants. (C) DLG-1(1-186)::GFP mislocalized into puncta along the junctional belt, with large regions of the junction lacking GFP. (G) DLG-1(1-468)::GFP localized along the lateral junction, although some gaps were present at the 1.5-fold stage of elongation. (K) DLG-1(1-710)::GFP localization was indistinguishable from wild type in dlg-1(ok318) embryos. (D,H,L) All constructs localized along the junctions of epidermal cells in ajm-1(RNAi) embryos. (E,I,M) Localization of DLG-1::GFP deletions in dlg-1(ok318);ajm-1(RNAi) double-mutant embryos. (E) DLG-1(1-186) was mislocalized away from the junction in the cytoplasm of epidermal cells in double mutants. (I) Localization of DLG-1(1-468)::GFP in dlg-1(ok318);ajm-1(RNAi) double mutants was similar to its localization in dlg-1(ok318) single-mutant embryos (G). (M) DLG-1(1-710)::GFP localization was indistinguishable from wild-type in dlg-1(ok318);ajm-1(RNAi) double mutants. Scale bar: 10 µm.





Right arrow Return to article