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Fig. 5. DLG-1 constructs truncated after the PDZ domains can localize AJM-1. Imaging of anti-AJM-1 staining and direct imaging of DLG-1::GFP deletions in terminally arrested dlg-1(ok318) embryos at approximately the twofold stage of elongation. (A-C) DLG-1(1-186)::GFP;dlg-1(ok318) mutant embryo. (A) DLG-1(1-186)::GFP mislocalizes into punctate aggregates in dlg-1 mutant embryos. (B) The construct is unable to rescue AJM-1 localization, which is mislocalized into puncta. (C) AJM-1 and DLG-1(1-186)::GFP puncta co-localize in dlg-1(ok318) mutants. (D-F) DLG-1(1-468)::GFP;dlg-1(ok318) mutant embryo. (D) DLG-1(1-468)::GFP is distributed along the junction in arrested embryos. (E) The construct rescues AJM-1 localization, which is well-distributed in arrested DLG-1(1-468)::GFP;dlg-1(ok318) embryos. (F) AJM-1 and the GFP construct co-localize. (G-I) DLG-1(1-710)::GFP;dlg-1(ok318) mutant embryo. (G) DLG-1(1-710)::GFP localizes to the junction in dlg-1(ok318) mutants. (H) AJM-1 is properly localized in DLG-1(1-468)::GFP;dlg-1(ok318) mutant embryos. (I) GFP and AJM-1 co-localize. (J-L) DLG-1( ${Delta}$ 17-116)::GFP;dlg-1(ok318) mutant stained. (J) DLG-1( ${Delta}$ 17-116)::GFP localizes to the junction in a mutant embryo (K), but fails to localize AJM-1 along the entire junction (L). Scale bar: 10 µm.

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