spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Membrane-binding and -tubulation by PX-BAR domains from SNX9, SNX18 and SNX30. (A) Epifluorescent micrographs of HeLa cells expressing myc-tagged PX-BAR domains from SNX9, SNX18 and SNX30. Scale bar: 10 µm. Note the long, myc-stained tubules formed by all three PX-BAR constructs. (B) Electron micrograph of membrane tubes generated by incubation of liposomes made from brain-derived lipids together with 20 µM of purified SNX18-PX-BAR protein. Inset shows 2.5x magnification of the indicated area. (C) Phosphoinositide-binding specificity of SNX18-PX-BAR analyzed by liposome co-sedimentation. SNX18-PX-BAR was incubated with liposomes made from brain-derived lipids (Brain), or with liposomes with defined lipid composition without (PS) or with indicated phosphoinositides (see Materials and Methods). After centrifugation, pellet (P) and supernatant (S) fractions were analyzed by SDS-PAGE and Coomassie-stained bands were quantitated by densitometry. (Below) The bars show the means from two experiments, with the maximum value indicated for each set. (D) Epifluorescent micrographs showing HeLa cells expressing myc-tagged SNX18-PX-BAR treated with ionomycin or wortmannin as indicated. Note the loss of membrane tubulation after ionomycin treatment. Scale bar: 10 µm. All PX-BAR constructs contained the contiguous Y domains (see Fig. 1A).





Right arrow Return to article