JCS023374 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Movie 1
• Movie 2 -

  Movies 1 and 2. Kip2p-3YFP associates with the plus ends of both growing and shrinking MTs. A functional and endogenously expressed Kip2p-3YFP fusion binds the MT plus ends during both MT growth and shrinking. 2D time-lapse; one frame every 2.5 seconds are played at a rate of approximately three frames/second.

• Movie 3 -

  Movie 3. Kip2p speckles moving towards the plus ends of MTs. Speckles of Kip2p-3YFP move along the indicated MT (arrow) towards the plus end. 2D time-lapse images acquired every 2.5 seconds are played at a rate of approximately three frames/second.

• Movie 4 -

  Movie 4. Bik1p speckles moving towards the plus ends of MTs. Speckles of Bik1p-3GFP move along the indicated MT (arrow) towards the plus end. 2D time-lapse images acquired every 2.5 seconds are played at a rate of approximately three frames/second.

• Movie 5
• Movie 6 -

  Movies 5 and 6. Dyn1p-3GFP associates with the plus ends of both growing and shrinking MTs. A functional and endogenously expressed Dyn1p-3GFP fusion binds to the plus ends of both growing and shrinking MTs. 2D time-lapse; one frame every 5 seconds is shown at a rate of approximately three frames/second.

• Movie 7 -

  Movie 7. Dyn1p speckles move towards the plus ends of MTs. Speckles of Dyn1p-3GFP move along the indicated MT (arrow) towards the plus end. 2D time-lapse images acquired every 5 seconds are played at a rate of approximately three frames/second.