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Figure 1


Fig. 1. Bik1p localization is Bim1p-dependent in Glu tubulin strains. (A) Images of representative cells from different backgrounds expressing Bik1p-3GFP from the native locus. From left to right: Bik1p-3GFP fluorescence (green); Spc42p-RedStar fluorescence (red); GFP and RedStar merged imaged. Arrows indicate the plus end of astral MTs. Scale bar: 3 µm. (B) Shows the quantification of fluorescence intensity at the plus ends (+ ends) of astral MTs and at the SPB. 25-35 cells were scored for each strain. The bars indicate the s.e.m. (C) Left: western blotting of whole-cell extracts with anti-GFP against Bik1p-3GFP and with YoL1/34 against {alpha}-tubulin. Right: ratio between the anti-GFP signal and the anti-{alpha}-tubulin signal obtained for four independent western blotting experiments. The bars indicate the s.e.m. Strains are: (1) TUB1, (2) TUB1 bim1{Delta}, (3) tub1-Glu and (4) tub1-Glu bim1{Delta}.





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