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Figure 2


Fig. 2. Kip2p in Glu tubulin strains. (A) Merged images of representative cells of the indicated diploid strains, expressing Kip2p-3YFP (green) and Spc42p-RedStar (red). Arrows indicates the plus ends of astral MTs. (B) Shows the quantification of Kip2p-3YFP fluorescence intensity at the plus ends (+ ends) of astral MTs in the indicated strains. 50 cells were scored for each strain. The bars indicate the s.e.m. (C,D) Kip2p-3YFP localizes to plus ends of both growing (C) and shrinking (D) MTs in Glu tubulin cells. (E) Illustration of Kip2p-3YFP speckle movement on Glu MTs. (F) Illustration of Bik1p-3GFP speckle movement on Glu MTs. For the diploid cells in C-F, arrows indicate the plus end of astral MTs, arrowheads indicate the SPB and double arrowheads highlight speckles moving towards the plus ends. Time is in seconds. Scale bars: 3 µm.





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