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Fig. 3. Dynein in Glu tubulin strains. (A) Merged images of representative cells expressing Dyn1p-3GFP (green) and Spc42p-RedStar (red). The diploid strains are indicated. Arrows indicate the plus end of astral MTs. Scale bar: 3 µm. (B) Shows the quantification of Dyn1p-3GFP fluorescence intensity at the plus ends of astral MTs in the indicated strains. 50 cells were scored for each strain. No signal was detectable in Bik1-deleted cells. The bars indicate the s.e.m. N.D., not detectable. Fluorescence is in arbitrary units. (C,D) Dyn1p-3GFP localizes to the plus ends of both growing (C) and shrinking (D) MTs in Glu tubulin cells. (E) Illustration of Dyn1p-3GFP speckle movement on Glu MTs. For the diploid cells shown in C-E, arrows indicate the plus end of astral MTs, arrowheads indicate the SPB and double arrowheads highlight speckles moving towards the plus ends. Time is in seconds. Scale bars: 3 µm. (F) Shows the quantification of Bik1p-3GFP fluorescence intensity at the plus ends of astral MTs in the indicated strain. Fluorescence is in arbitrary units. 50 cells were scored for each strain. Bars are s.e.m. N.D., not detectable.
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