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Figure 2


Fig. 2. The cytoplasmic tail of NgCAM contains an axonal sorting signal. (A,B) Cultured hippocampal neurons were transfected with DsRed (red) and either the LDLR-based chimera LexNct45-114 (A) or LexNct1-114 (B). The cell-surface population of the chimeras was stained with an antibody against an extracellular epitope of LDLR (green). In order to appreciate the difference in staining intensity on distal axons and on dendrites, single green channel insets are shown at the bottom. (C) Diagram of LDLR- or CD4-based chimeras carrying either the entire NgCAM cytoplasmic tail (Nct1-114) or the C-terminal half of the NgCAM cytoplasmic tail (Nct45-114). (D) Axon/dendrite polarity index (A/D PI) and the percentage of cells showing uniform, axonal or somatodendritic surface accumulation are shown for the constructs depicted in C (20-25 cells were analyzed). ***P<0.0001; statistically significant differences from the {Delta}CT backbones.





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