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Figure 2


Fig. 2. UAP56 is concentrated in RNA-splicing speckled domains and in the regions surrounding them. (A) CaSki cells were permeabilized, fixed and stained for both UAP56 and SRm160. Confocal microscopy showed that UAP56 was present in RNA-splicing speckled domains, in which it colocalized with SRm160, a well characterized RNA-splicing speckled-domain protein (Blencowe et al., 1998). In the regions around these domains, UAP56 was also present at high concentration, whereas SRm160 was much more confined to the speckled domain itself. Scale bar: 10 µm. (B) Fluorescence intensity profiles across six randomly selected RNA-splicing speckled domains from the nucleus in A showed that SRm160 was more confined to the speckled domains, whereas UAP56 concentrations extended outside RNA-splicing speckled domains. The x-axis represents pixel number along the line passing through the RNA-splicing speckled domain. The fluorescence intensities are plotted along the y-axis.





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