QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. Accurate positioning of Mug27 at anaphase II depends on proper FSM formation. (A) The homothallic haploid strain SOP069 (h⁹⁰ mug27⁺-gfp sad1⁺-mCherry spo15) was cultured in EMM2 with appropriate supplements and then transferred to EMM-N to induce meiosis. 10 hours later, cells at different stages of meiosis were stained with Hoechst 33342. The bar graphs were drawn by determining the Mug27-GFP localization in 69 spo15⁺ and 87 spo15 cells, and then plotting the frequencies of the indicated patterns. (B) The haploid strains SOP044 (h^– mug27⁺-gfp pat1-114; i) and SOP079 (h^– mug27⁺-gfp cdc11-123 pat1-114; ii) were monitored after shifting the temperature to induce meiosis. 5 hours later, cells at different stages of meiosis were stained with Hoechst 33342 (blue). Scale bars: 5 µm. (C) The bar graphs were drawn by determining the Mug27-GFP localization in 114 pat1-114 and 112 cdc11-123 pat1-114 cells, and then plotting the frequencies of the indicated patterns.

Return to article