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Figure 7


Fig. 7. Overexpression of sid2+ suppressed the abnormal phenotypes of mug27{Delta} cells. (A,B) The mug27+ (NP40-1C), mug27{Delta} (SOP023), mug27{Delta} pREP1-sid2-GFP (SOP115) and mug27{Delta} pREP1-GFP (SOP114) strains were cultured in EMM2 containing 1 µg/ml thiamine with supplements and then transferred to EMM2 without thiamine to induce the expression of Sid2-GFP or GFP proteins. Subsequently, 20 hours after the first medium replacement, the cells were transferred to fresh EMM2-N without thiamin to induce meiosis at 28°C. (A) DIC images of the asci are shown. Scale bar: 10 µm. Histogram shows spore diameter. The mean lengths of the spores and the standard deviations were analyzed by MetaMorph software (Universal Imaging Corp.). (B) Spore viability, measured by random spore analysis.





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