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Figure 4


Fig. 4. Dynamics of microneme and rhoptry biogenesis in T. gondii. Parasites expressing MIC3-GFP (left two columns, green) or ROP1-CAT-YFP (right two columns, green) were transfected with IMC1-mRFP (red) in order to track microneme or rhoptry biogenesis. Both organelles are located at the apical end of parasites during interphase (top row). As daughter cells begin to elongate (+40'), new micronemes and rhoptries, distinct from their maternal counterparts, associate with the pellicles of each daughter (white arrowheads). As daughter cells grow, the maternal micronemes and rhoptries (open arrowheads) disappear (+95' for micronemes, +80' for rhoptries) and residual material is packaged into residual bodies when daughters emerge (+140' for micronemes, +130' for rhoptries). Scale bars: 5 µm.





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