QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Dynamics of mitochondrial replication in T. gondii. (A) Time-lapse microscopy of parasites labeled with IMC1-YFP (green) and HSP60-RFP (red). During interphase, the single mitochondrion typically forms an elongated S or lasso shape. The mitochondrion branches at multiple locations during the early stage of daughter IMC formation (arrowheads, t=0'). These branches elongate and often surround the growing daughter IMCs (+60'), but rarely enter into the developing daughters until they begin to emerge from the mother (+110'). Once initiated, however, mitochondrial entry into the daughter cells is very rapid (+120'). A portion of the mitochondrion often remains within the residual material left behind when daughter parasites emerge after the completion of endodyogeny (+160'). Scale bar: 5 µm. (B) HSP60_L-YFP transgenics were fixed and labeled with anti-ACP antibody to reveal transient association of the apicoplast (red) and mitochondrion (green) during the G1 phase and the early stages of apicoplast elongation (before the initiation of daughter scaffold formation). Scale bar: 5 µm. (C) Apicoplast or mitochondrial association is also observed by transmission EM (image kindly provided by G. Warren, University of Vienna, Austria). Scale bar: 0.5 µm.

Return to article