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Figure 6


Fig. 6. Effects of oryzalin on the dynamics of organellar replication. In contrast to control cultures (left), T. gondii tachyzoites grown for 24 hours in 1 µM oryzalin (right) fail to organize daughter parasites (green), owing to the absence of subpellicular microtubules (Stokkermans et al., 1996; Morrissette and Sibley, 2002). Early organellar division events, including Golgi division (filled arrowheads), apicoplast elongation (open arrowheads), and ER and mitochondrial ramification still occur in the presence of oryzalin, but later events, such as nuclear, apicoplast, ER and mitochondrial division, do not. In all panels, DNA is labeled with DAPI (blue) and the inner-membrane complex scaffolding is labeled using IMC1-YFP (green). Red markers: row 1, GRASP-mRFP (Golgi); row 2, anti-ACP (apicoplast); row 3, P30L-mRFP-HDEL (ER); row 4, HSP60L-RFP (mitochondrion). Note the ER and mitochondrial material left behind in the central residual body after the emergence of daughter parasites (bottom two control panels on left). Scale bar: 5 µm.





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