JCS035873 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Two different siRNA oligonucleotide sequences against Moesin impaired HIV-1 infection. (A) Western blot analysis of specific silencing of endogenous expression of Moesin with two different siRNA sequences (Oligos 1M and 2M) 48 hours after siRNA-oligo nucleofection of CEM cells. Silencing of Moesin expression is quantified as the band intensity ratios to α-tubulin. Scrambled indicates the siRNA-oligo control. (B) Effect of silencing endogenous Moesin expression on HIV-1 viral infection in CEM cells by using two independent and different siRNA oligonucleotides (Oligo 1M and Oligo 2M). Data are from one representative experiment carried out in duplicate. Scrambled indicates the siRNA oligo control.

• Supplemental Figure S2 -

  Fig. S2. Time-course analysis for gp120-induced CD4/CXCR4 interaction in CEM cells. The CD4-CXCR4 association was enhanced after gp120/CD4 engagement, peaking at 60 minutes after gp120 (10 µg/ml) exposure. One representative experiment out of three is shown.