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Figure 2


Fig. 2. Endocytic defects in rbots mutants are fully rescued by the wild-type rbo gene. (A) Representative images of rbots and rbots/rbo2; rbo-egfp/rbo-egfp (rescue) larval NMJs loaded with FM1-43 dye after 10 minutes at 37°C (see Fig. 1, protocol 2). Scale bar: 10 µm. (B) Magnified loaded synaptic boutons in both genotypes. The wild-type rbo transgene rescues the rbots dye-loading defect. Scale bar: 5 µm. (C) Quantification of dye loading in wild-type (OR) and rbots compared with rescue animals.





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