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Fig. 6. RBO is required for Texas-red–avidin-tracer endocytosis in Garland cells. (A) RBO-GFP in rbo²/rbo²; rbo-egfp/rbo-egfp Garland cells is strongly expressed at the periphery. (B) Most RBO-GFP is tightly associated with the plasma membrane, but some RBO associates with internal organelles (arrows). (C) Wild-type (OR) cells loaded at 25°C (left) or following 10 minutes at 37°C (right). (D) rbo^ts at 25°C (left) is indistinguishable from control, but at 37°C (right) shows no dye internalization. (E) rbo^ts;syx^3-69 also shows a complete block of uptake at 37°C. (F) As positive control, shibire^ts shows a block at 37°C (right); this phenotype is indistinguishable from rbo^ts. Scale bars: 5 µm. (G) Quantification of fluorescence intensity underlying the plasma membrane.

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