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Figure 7


Fig. 7. Ultrastructure of Garland cells in wild type and in rbots mutants. (A) Transmission electron microscopy (TEM) images of wild-type and rbots Garland cells at 25°C. The cortical cell region extends ~3 µm, with many labyrinthine channels (thin black arrows). Numerous {alpha} vacuoles (a), longitudinal (white arrows) and transverse (thick black arrows) tubular elements, and coated vesicles and/or pits (arrowheads) are present in all section planes. Many sections also contain mitochondria (M) and β vacuoles (b). Wild type and rbots appear indistinguishable at 25°C. (B) Following 10 minutes at 37°C, the number of {alpha} vacuoles in rbots cells is reduced, with remnant vacuoles being larger and more irregular. Labyrinthine channels swell and distend with many vacuolar structures (*). Scale bar: 1 µm. (C) Higher-magnification images at 37°C. In rbots, very elongated labyrinthine channels extend into the cell; these channels contain many vacuolar structures (*). Thin black arrows show the origin of the labyrinthine channels at the basement membrane. Scale bar: 250 nm.





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