QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 8. Block in HRP endocytosis occurs in Garland cells in the absence of RBO. (A) Garland-cell endocytotic activity visualized using HRP uptake in wild type (OR) and rbo^ts after 10 minutes at 37°C. Many coated profiles of vacuoles (a) are labeled in wild type (left), among pre-existing unlabeled vacuoles (arrows). The mutant (right) shows no HRP uptake into vacuoles (a). N, nucleus. Scale bar: 1 µm. (B) Higher-magnification images of vacuoles (a) and labyrinthine channels (arrows). The mutant (right) shows an absence of labeling, with distended and swollen labyrinthine channels. Scale bar: 250 nm. (C) Tannic-acid impregnation shows labyrinthine channels that are continuous with the extracellular space. In rbo^ts, the channels are fused with many vacuolar inclusions. Scale bar: 250 nm. (D) Quantification of cell area. No change was observed in rbo^ts at 25°C compared with wild type, but a highly significant increase in cell area was seen at 37°C. (E) Quantification of loaded endosomes per section. A significant reduction in the number of loaded endosomes was observed in rbo^ts at 25°C compared with wild type, with near complete loss at 37°C.

Return to article