spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. MT1-MMP induces LRP1 processing. (A) Wild-type (MT1+/+) and MT1-MMP–/– (MT1–/–) VSMCs were cultured on polymerized collagen I for 48 hours in the presence of MMP inhibitor (GM6001, 10 µM) as indicated and treated with PDGF-BB (25 ng/ml) under serum-free conditions. Total cell lysates were analyzed by immunoblotting for both the {alpha}- and β-chains of LRP1. (B) The cells were treated with PDGF-BB for 48 hours. For the last 24 hours of PDGF-BB treatment, the cells were labeled with [35S]-methionine (50 µCi/ml) as indicated. Soluble LRP1 fragments were detected from the conditioned medium by immunoprecipitation with polyclonal rabbit anti-LRP{alpha} antibodies followed by immunoblotting with mouse monoclonal anti-LRP{alpha} antibodies (LRP{alpha}) or by autoradiography ([S35]-meth). Relative mobilities of the molecular-mass markers are indicated in kDa.





Right arrow Return to article