spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. LRP1 knockdown by siRNA rescues PDGF-BB-mediated dedifferentiation of MT1-MMP–/– VSMCs. (A) Wild-type (MT1+/+) and MT1-MMP–/– (MT1–/–) VSMCs were plated on type I collagen and transfected with control, β3-integrin (right panels) or LRP1 (left panels) siRNAs as indicated. The cells were then exposed to PDGF-BB (25 ng/ml) under serum-free conditions for 48 hours and lysed. The relative levels of LRP1, β3 integrin and PDGFRβ in the lysates were assessed by immunoblotting. β-tubulin served as a loading control. (B) The relative levels of calponin and SMA were assessed by immunoblotting. Similar results were obtained with two different siRNA oligomers targeting LRP1 [LRP(1) and LRP(2)] and β3 integrin. (C) Quantitative assessment of SMA and calponin protein levels normalized against β-tubulin. The data are expressed as relative values (mean ±1 s.d., n=3).





Right arrow Return to article